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. 2017 Feb 14;18(2):199. doi: 10.3390/ijms18020199

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Increased adhesion capacity and expression of some adhesion molecules in CD34+ cells in the LN. (A) MSC adhesion capacity evaluation of CFSE-labelled CD34+ cells obtained from the NN or the LN; cells were cultured with MSC for 4 h and counted by fluorescence microscopy. The percentage of adhered cells was calculated taking into account the total input of CD34+ cells (* p < 0.05). Labelling of (B) CD44, (C) CD49d, (D) CD49e, and (E) CD54 in freshly-isolated, NN, M+REH-CM, and LN CD34+ cells. Results are expressed as the median fluorescence intensity (MFI) from two independent experiments done in triplicates (n = 6) (ns: non-significant, * p < 0.05, ** p < 0.01, *** p < 0.001).

Increased adhesion capacity and expression of some adhesion molecules in CD34+ cells in the LN. (A) MSC adhesion capacity evaluation of CFSE-labelled CD34+ cells obtained from the NN or the LN; cells were cultured with MSC for 4 h and counted by fluorescence microscopy. The percentage of adhered cells was calculated taking into account the total input of CD34+ cells (* p < 0.05). Labelling of (B) CD44, (C) CD49d, (D) CD49e, and (E) CD54 in freshly-isolated, NN, M+REH-CM, and LN CD34+ cells. Results are expressed as the median fluorescence intensity (MFI) from two independent experiments done in triplicates (n = 6) (ns: non-significant, * p < 0.05, ** p < 0.01, *** p < 0.001).