Figure 5.

Reduced primitive colony forming units of CD34+ cells exposed to the LN. Colony-forming units of granulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), burst forming unit erythroids (BFU-E), CFU-erythroids (CFU-E), and CFU-granulocyte/macrophage (CFU-GM) by CD34+ co-cultured in the NN or the LN (LN established with REH cells, middle columns; LN established with primary ALL-B cells, right columns); absolute (left panels) and relative counts (right panels) for two different CD34+ samples were performed in duplicate (n = 4), panels (A) and (B), are shown. Results are expressed as the mean ± SEM (p-values: non-parametric one-way ANOVA; ns: non-significant, * p < 0.05, ** p < 0.01, *** p < 0.001).