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Effects of ID extract on Akt signaling pathway in breast cancer cells. Cells were treated with absence or presence of ID extract for 24 h and harvested to measure protein levels of Akt, p-Akt, and p-GSK-3β (phosphor-glycogen synthase kinase-3β) by western blotting. We used the housekeeping protein β-actin as a positive loading control in all experiments. The bars in the graphs represent the mean ± SD calculated from three independent experiments. * p < 0.05. ID: Ixeris dentata.
