Table 11.
Validated immune defense-associated target genes of miRNAs differentially expressed in RAW264.7 due to either PUFA supplementation (DHA or AA, 72 h, 15 µM) or combined PUFA supplementation plus LPS stimulation (24 h, 1 µg/mL) plus.
| miRNAs | Affected by | Target Genes | |
|---|---|---|---|
| Name | Entrez ID | ||
| miR-1a-3p | LPS, AA AA + LPS | Adenosine deaminase, RNA-specific (Adar) | 56417 |
| E26 avian leukemia oncogene 1, 5′ domain (Ets1) | 23871 | ||
| miR-1b-5p | LPS, DHA + LPS, AA + LPS | CD28 antigen (Cd28) | 12487 |
| miR-10b-5p | AA + LPS | A kinase (PRKA) anchor protein 8 (Akap8) | 56399 |
| DNA cross-link repair 1C (Dclre1c) | 227525 | ||
| E26 avian leukemia oncogene 1, 5′ domain (Ets1) | 23871 | ||
| Fas (TNFRSF6)-associated via death domain (Fadd) | 14082 | ||
| miR-21a-5p | DHA + LPS, AA + LPS | Fas ligand (Fasl) | 14103 |
| miR-30d-5p | DHA | CD300A molecule (Cd300a) | 217303 |
| Calcium-dependent protein kinase IV (Camk4) | 12326 | ||
| Colony stimulating factor 1 (macrophage) (Csf1) | 12977 | ||
| Interleukin 18 receptor 1 (Il18r1) | 16182 | ||
| miR-125a-3p | DHA + LPS, AA + LPS | TNF receptor-associated factor 6 (Traf6) | 22034 |
| V-set immunoregulatory receptor (Vsir) | 74048 | ||
| Transcription complex subunit NF-ATc4 (Nfatc4) | 73181 | ||
| Signal transducer activator of transcription 1 (Stat1) | 20846 | ||
| miR-126a-3p | LPS, DHA + LPS | POU domain, class 2, transcription factor 2 (Pou2f2) | 18987 |
| SAM domain and HD domain, 1 (SAMHD1) | 56045 | ||
| miR-146a-5p | DHA + LPS, AA + LPS | TNF receptor-associated factor 6 (Traf6) | 23034 |
| Interferon gamma (Ifng) | 15978 | ||
| Interleukin-1 receptor-associated kinase 1 (Irak1) | 16179 | ||
| Interleukin-1 receptor-associated kinase 2 (Irak2) | 108960 | ||
| miR-146b-5p | DHA + LPS | Nuclear factor kappaB p50 (Nfkb1) | 18033 |
| miR-155-5p | DHA + LPS, AA + LPS | MHC class I-related gene protein (Mr1) | 15064 |
| E26 avian leukemia oncogene 1, 5′ domain (Ets1) | 23871 | ||
| Fas (TNFRSF6)-associated via death domain (Fadd) | 14082 | ||
| Inhibitor of kappaB kinase epsilon (Ikbke) | 56489 | ||
| miR-199a-5p | AA + LPS | CD4 antigen (Cd4) | 12504 |
| miR-210-3p | DHA | Inositol polyphosphate-5-phosphatase D (Inpp5d) | 16331 |
| Lymphocyte cytosolic protein 2 (lcp2) | 16822 | ||
| Neural cell adhesion molecule 1 (Ncam1) | 17967 | ||
| miR-222-3p | DHA + LPS | 5-azacytidine induced gene 2 (Azi2) | 27215 |
| miR-297b-3p | DHA + LPS, AA + LPS | 2′-5′ oligoadenylate synthetase 3 (Oas3) | 246727 |
| CD28 antigen (Cd28) | 12487 | ||
| miR-345-3p | LPS, AA, DHA + LPS, AA + LPS | SLAM family member 8 (Slamf8) | 74748 |
| miR-365-3p | DHA | Macrophage MHC class I receptor 2 (Mfsd6) | 98682 |
| miR-375-3p | DHA | Janus kinase 2 (Jak2) | 16452 |
| Complement component 1 binding protein (C1qbp) | 12261 | ||
| Attractin (Atrn) | 11990 | ||
| miR-466h-3p | LPS, AA + LPS | Fas (TNFRSF6)-associated via death domain (Fadd) | 14082 |
| miR-542-3p | LPS, DHA, AA | CD1d1 antigen (Cd1d1) | 12479 |
| miR-582-3p | DHA + LPS | CD300 molecule like family member F (Cd300lf) | 246746 |
| miR-669a-3p | AA + LPS | CD47 antigen (Cd47) | 16423 |
| miR-669c-5p | DHA | Fyn proto-oncogene (Fyn) | 14360 |
| Chemokine (C-X-C motif) ligand 5 (Cxcl5) | 20311 | ||
| miR-669f-3p | AA, DHA + LPS, AA + LPS | 2'-5' oligoadenylate synthetase 3 (Oas3) | 246727 |
| CD200 receptor 1 (Cd200r1) | 57781 | ||
| Butyrophilin-like 9 (Btln9) | 237754 | ||
| Interleukin 17 receptor A (Il17ra) | 16172 | ||
| Interleukin 5 receptor, α (Il5rα) | 16192 | ||
| Lymphocyte antigen 96 (Ly96) | 17087 | ||
| Melanoma cell adhesion molecule (Mcam) | 84004 | ||
| Paired-Ig-like receptor A1 (Pira1) | 18722 | ||
| Tec protein tyrosine kinase (Tec) | 21682 | ||
| Selenoprotein S (Selenos) | 109815 | ||
| miR-1943-5p | LPS, DHA, DHA + LPS | TLR4 interactor with leucine-rich repeats (Tril) | 66873 |
| miR-3064-5p | DHA + LPS | AXL receptor tyrosine kinase (Axl) | 26362 |
| CD300A molecule (Cd300a) | 217303 | ||
| miR-3074-1-3p | DHA | Leukocyte surface antigen CD47 (Cd47) | 16423 |
| miR-3079-5p | LPS, DHA | Fas (TNFRSF6)-associated via death domain (Fadd) | 14082 |