Figure 5.

Inhibition of tunicamycin-induced epithelial mesenchymal transition by losartan. Proximal tubular cells were incubated with tunicamycin (TM, 0.2 µM) with or without losartan (losar, 10 µM) for 24 h. Expression of α-SMA (α-smooth muscle actin) (A) and E-cadherin (B) was examined by Western blot analysis. The relative densities of the bands for α-SMA and E-cadherin were normalized to those for actin for standardization. Representative blots and quantitative analysis from three independent experiments were shown. Results were expressed as n-fold increase over control as mean ± S.E. #: p < 0.05 vs. con (control); ##: p < 0.05 vs. TM.