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. 2017 Feb 8;18(2):315. doi: 10.3390/ijms18020315

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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VPA plays a neuroprotection role in primary cortical neurons and affects matrix metalloproteinase (MMP) family protein activation. (A) The protocol of the experiment in primary cortical neurons; (B) Immunofluorescence of primary cortical neurons after TG treatment. β-IIItublin (green); NeuN (red); the nuclear was labeled by DAPI (blue). Magnification was 60×; (C) The mean neurite length of primary cortical neurons, ^# p < 0.05 versus the TG group; (D,E) Gelatin zymography for the cell medium in primary cortical neurons and neuronal cells line SH-SY5Y. Data are the mean values ± SEM, n = 3.