Table 2.
Fatty acid content of not supplemented (NS) and supplemented cells after 6 h supplementation. Data were obtained by gas chromatography (GC) analysis, and are expressed as µg FA/106 cells. Data in each group are means ± standard deviation (SD) of five samples derived from three independent experiments. Statistical analysis was conducted with the one-way ANOVA (22:6n-3 p < 0.001; UI p < 0.001; n-3/n-6 p < 0.001; ΣPUFA p < 0.001) using Dunnett’s post-test to compare NS and supplemented cells (** p < 0.01; *** p < 0.001).
| FA | NS | 70 µM PRO | 20 µM PCA | 50 µM DHA | 50 µM DHA + 70 µM PRO | 50 µM DHA + 20 µM PCA |
|---|---|---|---|---|---|---|
| 3:0 | 0.06 ± 0.07 | 0.13 ± 0.09 | 0.03 ± 0.03 | 0.09 ± 0.10 | 0.04 ± 0.07 | 0.03 ± 0.04 |
| 14:0 | 1.30 ± 0.62 | 1.07 ± 0.64 | 1.11 ± 0.53 | 1.91 ± 1.23 | 1.63 ± 0.13 | 1.61 ± 0.09 |
| 16:0 | 22.37 ± 7.28 | 21.71 ± 4.67 | 21.08 ± 3.40 | 24.71 ± 8.04 | 26.6 ± 4.85 | 23.65 ± 1.32 |
| 16:1n-7 | 1.63 ± 0.28 | 1.85 ± 0.76 | 1.93 ± 0.39 | 2.04 ± 0.62 | 2.21 ± 0.59 | 2.21 ± 0.15 |
| 18:0 | 19.95 ± 7.96 | 18.9 ± 3.58 | 17.70 ± 2.65 | 19.90 ± 6.87 | 21.87 ± 3.89 | 19.19 ± 2.41 |
| 18:1n-9 | 5.85 ± 1.13 | 7.30 ± 2.47 | 6.76 ± 1.76 | 7.74 ± 2.59 | 7.48 ± 2.17 | 6.81 ± 1.00 |
| 18:1n-7 | 6.84 ± 1.09 | 7.60 ± 2.28 | 7.56 ± 1.64 | 6.30 ± 1.46 | 7.65 ± 2.36 | 6.84 ± 0.84 |
| 18:2n-6 | 0.26 ± 0.11 | 0.27 ± 0.08 | 0.26 ± 0.06 | 0.36 ± 0.28 | 0.33 ± 0.05 | 0.28 ± 0.05 |
| 18:3n-3 | 0.37 ± 0.13 | 0.32 ± 0.13 | 0.31 ± 0.15 | 0.53 ± 0.25 | 0.44 ± 0.11 | 0.37 ± 0.05 |
| 20:4n-6 | 6.19 ± 1.36 | 7.43 ± 1.96 | 7.52 ± 0.89 | 6.77 ± 1.37 | 8.93 ± 1.47 | 7.94 ± 1.32 |
| 20:5n-3 | 0.25 ± 0.06 | 0.24 ± 0.05 | 0.29 ± 0.21 | 0.32 ± 0.16 | 0.53 ± 0.52 | 0.24 ± 0.02 |
| 22:6n-3 | 0.92 ± 0.46 | 0.61 ± 0.37 | 0.67 ± 0.23 | 6.16 ± 1.07 *** | 6.98 ± 2.48 *** | 6.31 ± 0.80 *** |
| UI | 45.87 ± 9.08 | 50.97 ± 13.88 | 51.32 ± 7.67 | 81.99 ± 16.46 *** | 97.38 ± 22.80 *** | 86.17 ± 4.30 *** |
| n-3/n-6 | 0.24 ± 0.09 | 0.15 ± 0.02 | 0.16 ± 0.06 | 1.00 ± 0.15 *** | 0.87 ± 0.28 *** | 0.87 ± 0.25 *** |
| ΣSFA | 43.68 ± 15.7 | 41.81 ± 8.68 | 39.92 ± 6.29 | 46.61 ± 16.08 | 50.13 ± 8.88 | 44.48 ± 3.61 |
| ΣMUFA | 14.32 ± 1.06 | 16.75 ± 5.45 | 16.25 ± 3.70 | 16.09 ± 4.59 | 17.34 ± 5.09 | 15.87 ± 1.70 |
| ΣPUFA | 8.00 ± 1.91 | 8.87 ± 2.22 | 9.05 ± 1.07 | 14.14 ± 2.87 ** | 17.21 ± 3.41 *** | 15.15 ± 0.88 *** |
| Total FA | 66.00 ± 16.66 | 67.42 ± 15.73 | 65.21 ± 10.15 | 76.83 ± 23.03 | 84.69 ± 17.18 | 75.49 ± 4.13 |
DHA: docosahexaenoic acid; FA: fatty acids; MUFA: monounsaturated fatty acids; NS: not supplemented; PCA: protocatechuic acid; PRO: propionic acid; PUFA: polyunsaturated fatty acids; SFA: saturated fatty acids; UI: unsaturation index.