Figure 2.
(A) Fluorescence images showing the nuclear morphology of HCT116p53−/− and HCT116p21−/− cells before and after irradiation. Nuclear content (DAPI) is shown in white. All images were acquired at the same magnification; (B) Proportion of MNGCs arising in cultures of the indicated cell lines following radiation exposure; Bars, SE (Standard error). At ~1 week post-irradiation, some dishes contained regions with colonies consisting of heterogeneous cells. The image of a small colony is shown in (C); Cells in such colonies were not used for the data presented in panel (B).