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. 2017 Feb 8;18(2):360. doi: 10.3390/ijms18020360

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Viability of MNGCs created in HCT116p53−/− cultures at seven days after radiation exposure (8 Gy), evaluated by the PI-exclusion assay. The two images were taken from the same field, before (left image) and after (right image) addition of methanol to the culture medium to permeabilize the cells. Circles mark viable giant cells. The lower panel shows quantitative data for HCT116p53−/− and HCT116p21−/− cultures at different times post-irradiation. Bars, SE; (B) Viability of the same cultures evaluated by the TB-exclusion assay.