Figure 3.

The impact of mercuric chloride on Twist2 expression. In the top panel, 20 mon old rats were challenged with mercuric chloride and Twist2 expression was assessed 2 or 10 day after injury. Each data point represents the mean + SEM of 3–4 animals, * indicates a significant difference from young control (4 mon). In the bottom panel, DCT209 cells were challenged with 5 µM mercuric chloride for 24 h and Twist2 expression assessed by immunofluorescence (green staining), scale bar = 50 µm; similar results were seen in two replicate experiments.