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. 2017 Feb 10;18(2):370. doi: 10.3390/ijms18020370

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Deguelin inhibits pancreatic cancer cell growth and induces apoptosis. (A,B) Mia PaCa-2 and Panc-1 cells were treated with deguelin at indicated concentrations or time points. Cell Counting Kit-8 assay was performed to evaluate cell proliferation; (C) Representative images from the clonogenic assays. Mia PaCa-2 and Panc-1 cells were incubated with indicated concentrations of deguelin and cultured for 14 days. Each bar represents means ± Standard Deviation (SD) of three separate experiments (right); (D) Significant increase in apoptosis after cells were treated as described in (C). Apoptotic cells (Annexin V⁺) were analyzed and results are presented as the mean ± SD (right); (E) Western blot analysis for cleaved PARP and cleaved caspase-3 was performed on lysates from cells treated as described in (C). GAPDH from a similarly loaded gel is shown as loading control. * p < 0.05, ** p < 0.01 versus the control group. GAPDH: glycerinaldehyde-3-phosphat-dehydrogenase.

Deguelin inhibits pancreatic cancer cell growth and induces apoptosis. (A,B) Mia PaCa-2 and Panc-1 cells were treated with deguelin at indicated concentrations or time points. Cell Counting Kit-8 assay was performed to evaluate cell proliferation; (C) Representative images from the clonogenic assays. Mia PaCa-2 and Panc-1 cells were incubated with indicated concentrations of deguelin and cultured for 14 days. Each bar represents means ± Standard Deviation (SD) of three separate experiments (right); (D) Significant increase in apoptosis after cells were treated as described in (C). Apoptotic cells (Annexin V⁺) were analyzed and results are presented as the mean ± SD (right); (E) Western blot analysis for cleaved PARP and cleaved caspase-3 was performed on lysates from cells treated as described in (C). GAPDH from a similarly loaded gel is shown as loading control. * p < 0.05, ** p < 0.01 versus the control group. GAPDH: glycerinaldehyde-3-phosphat-dehydrogenase.