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. 2017 Feb 10;18(2):370. doi: 10.3390/ijms18020370

Figure 2.

Figure 2

Deguelin induces incomplete autophagy in pancreatic cancer cells. (A) Mia PaCa-2 and Panc-1 cells were treated with 25 μM of deguelin for the indicated time points. Protein expression levels of LC3-II and p62 were measured by western blot; (B) Mia PaCa-2 and Panc-1 cells were treated with deguelin at the indicated concentrations for 24 h, with or without chloroquine (CQ). Cell lysates were analyzed by western blot; (C) Mia PaCa-2 and Panc-1 cells were treated with deguelin (25 μM) and/or CQ (10 μM) for 24 or 48 h and cell lysates were analyzed by western blot. GAPDH from a similarly loaded gel is shown as loading control.