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. 2017 Feb 10;18(2):372. doi: 10.3390/ijms18020372

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell migration and CSC associated genes were reversed by AXL overexpression in 1XM. (A) The photograph of colony selection and AXL overexpression confirmed by Western blotting. Migration assay of 1XM-neo (a) and 1XM-AXL (b) performed using wound healing assay (B), with BP administered at various doses in 24-h treatment, and the quantifications as (C). (D,E) When AXL was overexpressed in 1XM, AXL, EZH2, Oct4, Sox2 and CD133 were recovered. The photographs of (A,B) were taken under a light microscope at a magnification of ×40.