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. 2017 Feb 10;18(2):373. doi: 10.3390/ijms18020373

Figure 3.

Figure 3

Figure 3

Effects of ESL on the expression of KLF2 and VCAM-1 proteins and atherogenic genes, and phosphorylation of mitogen-activated protein kinases (MAPKs) in 7-ketocholesterol (7-KC)-stimulated HUVECs. (A) The cells were pretreated with or without ESL for 2 h, and then stimulated with 7-KC in a dose- and time-dependent manner; (B) The mRNA levels of the indicated targets were determined by real-time qRT-PCR as described in the Materials and Methods Section. # p < 0.01 vs. untreated group. * p < 0.05 and ** p < 0.01 vs. the 7-KC-treated group; (C) The cells were stimulated with 7-KC or TNF-α for the indicated incubation times; (D) Cells were pretreated with or without ESL for 2 h, and then stimulated with 20 μg/mL 7-KC for 30 min. The protein levels were analyzed by Western blot with the respective primary antibodies and the corresponding phospho-specific antibodies. The total MAPK level was used as the internal control.