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. 2017 Feb 14;18(2):409. doi: 10.3390/ijms18020409

Figure 6.

Figure 6

SGT1 (suppressor of G2 allele of skp1) is required for PITG_22798-induced cell death. (A) Representative symptoms of PITG_22798-induced cell death on SGT1- and HSP90-silenced N. benthamiana leaves at 6 d post-infiltration. (B) RT-PCR was done to test the silencing efficiency of SGT1 and HSP90. EF1α was used to confirm equal total RNA amounts among samples. (C) Quantification of cell death and ion leakage on SGT1- and HSP90-silenced N. benthamiana leaves. Cell death differences were based on three independent experiments scored at 6 dpi (upper panel). Ion leakage was measured at 0 and 6 dpi (lower panel). Results were obtained from 120 infiltration sites based on five independent experiments. Values are means ± SD. Asterisks denote values significantly different from control TRV: GFP (p < 0.01, t-test).