Figure 2.

Suppression of CCAR1 induces apoptotic cell death in gastric cancer cells. (A) Cell cycle distribution of propidium iodide (PI)-labeled cells was analyzed by flow cytometric analyses. The peaks in the illustration correspond to the subG₁, G₁, S, and G₂/M phases of the cell cycle; (B) Statistical analysis of cell cycle phase distribution. Data are presented as means ± SD of three independent tests. *** p < 0.001 versus control; (C) Expression of the apoptosis-related proteins, poly (ADP-ribose) polymerase 1 (PARP-1) and Caspase-3, and their cleaved patterns in gastric cancer cell lines (AGS and MKN28) without infection, infected with control shRNA lentiviruses (shNullT), and infected with two separate CCAR1-specific shRNA lentiviruses, were analyzed by western blot analysis. Actin was used as the loading control.