Figure 6.
Expression and production of FN on HHDPCs treated with e-CAF. (A) Immunofluorescent assay on HDFs and HHDPCs treated or not (control) with e-CAF (1 and 10 µg/mL) for seven days. Pictures show specific staining of FN on HDFs and HHDPCs under the different experimental conditions by using specific antibodies. Scale bar: 50 μm. DAPI, 4′-6-diamidino-2-phenylindole; and (B) ELISA assay on three-day supernatants from HDFs and HHDPCs cultures. The graph shows the media and SD of FN (µg/105 cells) from triplicates in HDFs and HHDPCs supernatants under the different experimental conditions in a representative experiment; * p < 0.05.