. 2017 Feb 21;18(2):464. doi: 10.3390/ijms18020464

Table 1.

Gene primers tested in the study.

Gene Primer	Sequence	Annealing Temperature
mlh-1	Forward GAGAAGACGATGATGTGGA	50.3 °C
mlh-1	Reverse GAGATGGTAGAGGGAGGTG	50.3 °C
cyb-3	Forward ATGGTCTCACAATCCCGTC	62.9 °C
cyb-3	Reverse GTGGCATCACCTCCTCTCC	62.9 °C
cdc-25.2	Forward GCCAGGTGCGTCTGTTCG	54.2 °C
cdc-25.2	Reverse CGTTTCCGCTGCTGTAGG	54.2 °C
glp-1	Forward AACTGTTGTCGCTGGTGT	50.0 °C
glp-1	Reverse TCTCTTGGTATTGGGGTG	50.0 °C
ced-3	Forward ACGGGAGATCGTGAAAGC	53.0 °C
ced-3	Reverse AGAGTTGGCGGATGAAGG	53.0 °C
ced-4	Forward AGTCACTCGCAATGGCTCT	57.5 °C
ced-4	Reverse GCTGATGAACGACGGAAT	57.5 °C
ced-9	Forward AAAGGCACAGAGCCCACC	50.0 °C
ced-9	Reverse CGTTCCCATAACTCGCATC	50.0 °C
folt-1	Forward TCCATTCCTCACTCCGTTTCTA	60.4 °C
folt-1	Reverse GCATCTGCCATACTCCTTTACC	60.4 °C
spe-10	Forward TTTTATTGTCGGCGGAGTGT	57.8 °C
spe-10	Reverse CGATGACTGCGAACTTTGAG	57.8 °C
spe-15	Forward GGAGTTTTGGATGTCGCTGGTT	60.4 °C
spe-15	Reverse GCTCTCTGGGTGAAATGTTGGA	60.4 °C
act-1	Forward ATGTGTGACGACGAGGTT	60.4 °C
act-1	Reverse GAAGCACTTGCGGTGAAC	60.4 °C