Fig. 5.
Time-lapse imaging of receptor insertion into the surface plasma membrane in cortical neurons. (A) The insertion of BBS-GFP-GluR2 into the surface membrane in the transfected neurons was observed after preincubating neurons with unlabeled BTX, followed by labeling with rhodamine-BTX. The clusters of receptors newly inserted in the surface could be seen in the dendrites and spines. Arrowheads show individual clusters of BBS-GFP-GluR2. (B) Quantification of signal intensity of individual clusters (n = 19).