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. 2017 Mar 8;69(4):625–632. doi: 10.1161/HYPERTENSIONAHA.116.08507

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© 2017 The Authors.

Hypertension is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 3. — A, Representative raw data trace showing fluorescence ratio of fura-2AM to assess the effect of SERCA (sarco-endoplasmic reticulum calcium ATPase) gene transfection on endoplasmic reticulum (ER) Ca²⁺ handling. The effect of caffeine (10 mmol/L for 30 s at 5 min; to empty ER Ca²⁺ store) and thapsigargin (1 μmol/L thapsigargin from 5 min; to prevent ER Ca²⁺ reuptake) evoked intracellular Ca²⁺ changes in isolated Sprague–Dawley (SD) stellate neurons transfected with mCherry empty or SERCA. B, Group mean data of [Ca²⁺]i in response to caffeine (empty: n=35; SERCA: n=45) and thapsigargin (empty: n=33; SERCA: n=42). **P<0.01. C, Representative raw data trace showing the effect mCherry empty or SERCA2a gene transfection had on mitochondrial Ca²⁺ uncoupling by carbonylcyanide-p-trifluoromethoxyphenylhydrazine (FCCP; 1 μmol/L) in isolated SD rat stellate ganglia neurons. D, Group mean data of [Ca²⁺]_i in response to FCCP (empty: n=22; SERCA: n=22). ****P<0.0001.