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. 2004 Nov 24;101(49):17033–17038. doi: 10.1073/pnas.0407902101

Fig. 4.

Fig. 4.

Mammalian GBP specificity. C-type lectin (DC-SIGN): DC-SIGN-Fc chimera (30 μg/ml) detected by secondary goat anti-human-IgG-Alexa 488 antibody (10 μg/ml) bound selectively to α1–2- and/or α1–3/4-fucosylated glycans and to Manα1–2 glycans. Siglec (CD22): CD22-Fc chimera (10 μg/ml) precomplexed with secondary goat anti-human-IgG-Alexa 488 (5 μg/ml) and tertiary rabbit anti-goat-IgG-FITC (2.5 μg/ml) antibodies bound exclusively to Neu5Acα2–6Gal-glycans. Galectin (galectin-4): Human galectin-4-Alexa 488 (10 μg/ml) evaluated with glycans printed at 100 and 10 μM bound preferentially to blood group glycans.m