Table 1. Valency of GBPs analyzed with the glycan array.

Category	GBP	Valency	2° Ab	3° Ab	Final
Plant lectin	Con A-FITC	4			4
Plant lectin	ECA-FITC	2			2
Human C type	DC-SIGN-Fc*	2			2
Human siglec	CD22-Fc	2	α-hIgG-F	α-gIgG-F	8
Human galectin	Galectin-4-AF488	2			2
Human IgG	Anti-CD15-FITC	2			2
Human IgG	2G12	2	α-hIgG-AF		4
Human IgG/A/M	Serum†	2			2
Bacterial GBP	Cyanovirin‡	2			2
Viral GBP	Influenza HA (H3)	3	α-HA-AF	α-mIgG-AF	12
Intact virus	Influenza (PR8)§	500	α-PR8	α-rIgG-AF	500

Ab, antibody; F, FITC; AF, Alexa Fluor 488; HA, hemagglutinin.

^*After binding of DC-SIGN, binding was detected by overlay with anti-human IgG-AF488.

^†After binding of serum diluted 1:25 with PBS, binding was detected by overlay with goat anti-human IgG/M/A-Biotin (1:100) (Pierce) followed by streptavidin-FITC (1:100).

^‡After binding of CVN, binding was detected by overlay with polyclonal rabbit anti-CVN IgG-AF488 followed by anti-rabbit-IgG-FITC.

^§After binding of virus, binding was detected by overlay with rabbit anti-PR8 followed by goat anti-rabbit-IgG-AF488.