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Nicotine distribution in the (A) serum and (B) brain of immunized mice. Serum and brain tissues of mice were collected 4 min after administration of 0.03 mg/kg nicotine subcutaneously on Day 54, and nicotine contents in tissues were analyzed. * and ** indicate significant differences compared to the negative control group, * p<0.05, ** P<0.01; # P<0.05.
