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Determination of Nic-hapten density, Nic-BSA thiolation grade and AE and LC of Nic-BSA to NsL NPs.
| Molar ratio of additive (BSA: Nic:Traut’s reagent: DSPE-Mal-PEG2000) | Approximate no. of amino groups used | Approximate no. of thiol groups used | AE (%) |
LC (%) |
|---|---|---|---|---|
| 1:50:200:4 | 15.1 (15) | 5.69 (5) | 50.4 ± 1.0 | 21.7 ± 2.4 |
| 1:50:200:8 | 15.1 (15) | 5.69 (5) | 65.8 ± 1.4 | 25.3 ± 4.6 |
Notes: Results are expressed as means (SD) for n = 3. AE: association efficiency; LC: loading capacity.
