Figure 3.

βHB increases the expression of enterocyte and goblet cell markers in LS174T cells. LS174T cells were treated with βHB (10 mM) for 48 h. Total RNA was extracted, and IAP (enterocyte marker) and KRT20 (enterocyte marker), MUC2 (goblet cell marker), p21^Waf1 and CDX2 mRNA expression was assessed by real-time RT-PCR. (n=3, data represent mean±S.D.; *P<0.01 versus control). Data are from one of three independent experiments with similar results. (b) LS174T cells were treated with βHB at various dosages for 48 h. Cells were lysed and western blot analysis was performed using antibodies against p21^Waf1, CDX2, H3K9ac, MUC2, KRT20 and β-actin. The images are representative of three independent experiments