Figure 3.

A1^−/− mice exhibit normal T cell immune responses to chronic infection with LCMV docile. Wild-type and A1^−/− mice were infected i.v. with 2 × 10⁶ pfu LCMV docile and spleens were analysed 8 days post infection by flow cytometry. (a) Total numbers of CD8⁺ as well as CD4⁺ T cells and B cells were assessed by cell counting and flow cytometric analysis. (b) CD8⁺ T cells were further analysed for CD62L⁺CD44⁻ naive, CD69⁺ activated, CD44⁺CD62L⁺ central memory (Tcm) like, CD44⁺CD62L⁻ effector memory (Tem) like T cells. (c) CD8⁺ T cells were also analysed for CD127-KLRG1⁺ short-lived effector cells (SLEC) and CD127⁺KLRG1⁻ memory precursor effector cells (MPEC). (d) The numbers of GP33⁺ low-affinity antigen-specific, or NP396⁺ high-affinity antigen-specific CD8⁺ T cells were assessed. (e) Spleen cells from infected mice were cultured in the presence of adenovirus, GP33, or NP396 antigen overnight and intracellular staining for IFN-γ was performed and cells analysed by flow cytometry. (f) LCMV titres were calculated from spleens and livers of infected mice 8 days post infection. Bars represent means±S.E.M. (n=4)