Fig. 1.

Protein secretion by C. pinensis grown in liquid culture containing three different carbon sources. Media contains glucose, konjac glucomannan or carob galactomannan as sole carbon course at 0.5% final concentration. Protein secretion was measured using the Bradford assay to determine protein concentration in the media at various time-points throughout growth. Solid lines: protein concentration in growth medium (g L⁻¹). Dashed lines: protein concentrations normalised for cell density by dividing by A₆₀₀. Error bars represent one standard deviation from the mean. Compared to glucose and CGM, growth on KGM reached the highest final OD, and the growth curve adhered most closely to the classical three-stage growth profile of a bacterial culture. The glucomannan liquid cultures also showed the highest concentration of protein in the growth medium, as measured by the Bradford assay [3].