Subject area	Microbiology, Biochemistry
More specific subject area	Bacterial protein secretion and carbohydrate deconstruction
Type of data	Tables and figures
How data was acquired	The analysis utilised a nanoHPLC-MS/MS system consisting of a Dionex Ultimate 3000 RSLCnano (Thermo Scientific, Bremen, Germany) connected to a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) with a nano-electrospray ion source.
Data format	Raw, analysed
Experimental factors	The bacterium was grown on agarose plates containing one of three tested substrates (glucose, glucomannan and galactomannan). Secreted proteins were collected from the solid medium following trypsin hydrolysis performed within the agarose medium. Samples were collected at three time-points during growth.
Experimental features	Three biological replicates were collected for each time-point sample. Proteins were hydrolysed by trypsin within the solid medium. Released peptides were then prepared for analysis by mass spectrometry. Two technical replicate experiments were performed for each sample. Raw data was normalised and analysed using the Max Quant programme, with quantification performed using the MaxLFQ algorithm.
Data source location	Proteomic data were collected in-house at the Norwegian University of Life Sciences, Ås, Norway
Data accessibility	Data is with the article and at PRIDE: PXD004305.