Table 2.
Screening secretion of cytokines of CC-sensitive (CC-S) and CC-resistant (CC-R) women with PCOS.
| Cytokines | Fluorescence intensity | P-value | |
|---|---|---|---|
| CC-S (n=4) | CC-R (n=4) | ||
| Angiopoietin-2 | 299.03±109.37 | 160.35±12.56 | 0.05 |
| uPAR | 501.69±27.76 | 372.13±93.69 | 0.04 |
| TIMP-1 | 2846.80±349.61 | 2143.04±295.11 | 0.02 |
| sTNFR2 | 2214.21±203.90 | 1696.79±254.46 | 0.02 |
| Acrp30 | 68648.05±1721.00 | 65635.77±1280.93 | 0.03 |
| CXCL-16 | 1107.60±130.55 | 1549.45±194.84 | 0.01 |
| ErbB3/Her3 | 3459.52±329.81 | 2693.26±266.28 | 0.01 |
Fasting plasma of each subject was incubated with the sets of cytokine antibody microarrays like those shown in Figure 1. Cytokine levels were measured by laser scanner (GenePix 4000B Microarray Scanner), normalized to internal positive controls and expressed as fluorescence intensity. Only the cytokines, which differed between the two groups of subjects, are shown in the table. Seven cytokines were differentially secreted between CC-S and CC-R women. Values are presented as means ±SD for 4 subjects per group. uPAR – urokinase receptor; TIMP-1 – tissue inhibitor of metalloproteinase-1; sTNF-R2 – soluble tumor necrosis factor receptor-2; CXCL-16 – chemokine (C-X-C motif) ligand 16; ErbB3/Her3 – human epidermal growth factor receptor 3.