Table 4. Overview on lipid MS1 features detected in C. elegans samples in the two respective ion modes with reliable LipidFrag results.

Ion mode	No. of cluster	With accurate mass annotation	With MS/MS	Manually identified in standards	Reliable LipidFrag (FCP cut-off)
Pos	1655	1297	685	65	• 108 (0.7) • 106 (0.8) • 100 (0.9) • 98 (0.95)
Neg	505	358	228	52	• 45 (0.7) • 43 (0.8) • 43 (0.9) • 40 (0.95)