Table 3.

Technical Validation of the NIC Assay

Technical Validation Step	Results
Detection range (LLOD-ULOD)	3.1-260 nM
LLOQ	3.2 nM
Intraassay variation	9%
Interassay variation	14%
Dilution recovery in serum⁎	104% (80-115%)
Dilution recovery in plasma EDTA⁎	92% (81-100%)
Spiking recovery in serum⁎	91% (83-105%)
Spiking recovery in plasma EDTA⁎	94% (80-102%)
Freeze-thaw recovery in serum⁎	95% (89-101%)
Freeze-thaw recovery in plasma EDTA⁎	85% (78-101%)
Analyte stability serum 24 h, 4°C/20°C⁎	88% (80-94%)/82% (71-87%)
Analyte stability plasma EDTA 24 h, 4°C/20°C⁎	90% (86-94%)/98% (95-105%)
Interference biotin, low/high⁎	98%/118%
Interference lipemia, low/high⁎	93%/81%
Interference hemoglobin, low/high⁎	105%/101%

^⁎Percentages are reported as mean with range shown in brackets.