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. 2017 Mar 2;12:274–284. doi: 10.1016/j.redox.2017.02.024

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — MLN64 overexpression increases mitochondrial cholesterol content in mice liver (A) MLN64 immunoblot. Total liver extracts (50 μg protein/lane) from Ad.E1Δ and Ad.MLN64 mouse livers 48 h after infection, run on 12% SDS-PAGE, transferred to PVDF membranes. (B) Mitochondria were isolated from Ad.E1Δ and Ad.MLN64 mouse livers 48 h after infection by Percoll density ultracentrifugation. The purity of mitochondria was confirmed by determining the distribution of specific markers such as CoxIV (mitochondria), Lamp1 and cathepsin B (lysosome) using immunoblot analysis. (C) Mitochondrial cholesterol content was quantified in the pure mitochondria fractions using HPLC. * Indicates statistically significant differences (p<0.05). (n Ad.E1Δ=5; n Ad.MLN64=5).