Fig. 3.

MLN64 overexpression produces mitochondrial dysfunction in hepatocytes (A) 48 h after Ad.E1Δ and Ad.MLN64 infection HepG2 cells were stained with MitoSOX Red to measure superoxide production and with Mitotracker Green to stain mitochondria respectively. Scale bar: 20 µm. (B) The graph shows the fluorescence intensity of MitoSOX as indicative of superoxide production. * p<0.05 (n=5). (C) MLN64 (green) and Mitotracker Red (red) staining of HepG2 cells 48 h after adenoviral infection. MMP levels were determined with Mitotracker red (MTred) staining and confocal imaging. MLN64 immunofluorescence was performed after cells were stained with MTred. The graph shows the quantification of MTred fluorescence intensity. * p<0.05 (n=5). Scale bar: 20 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)