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. 2017 Mar 9;12(3):e0173352. doi: 10.1371/journal.pone.0173352

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© 2017 Chapman et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — p24 Gag production by: (A) DNA vaccines in HEK cells. HEK cells were transfected with 4μg of DNA-Gag^M or DNA-Gag^N and samples taken at the indicated time points. (B) Recombinant MVA in BHK-21 and HeLa cell lines. Permissive (BHK-21) and non-permissive (HeLa) cell lines were infected at an MOI of 0.1 with MVA-Gag^M and samples taken at the indicated time points. Gag p24 was detected using an ELISA assay.