Figure 5.
Medium supernatant of HepG2 cells, which were treated with BRL37344, decreases TC and CE contents, increases FC content, and prompts cholesterol efflux in macrophages. (A–C) Enzymatic assay analysis of TC, FC, and CE (CE = TC – FC) was performed in macrophage foam cells, and (D) cholesterol efflux rate was tested using [3H] cholesterol-loaded macrophages that were incubated with the supernatant of HepG2 cells. Vehicle group: lipid-loaded RAW264.7 cell without any supernatants treatment; control supernatant group: lipid-loaded RAW264.7 cell cocultured with supernatants from HepG2 cells with DMSO treatment; low-dose BRL37344 supernatants group: lipid-loaded RAW264.7 cell cocultured with supernatants from HepG2 cells with 10−6 mol/L BRL37344 treatment; high-dose BRL37344 supernatants group: lipid-loaded RAW264.7 cell cocultured with supernatants from HepG2 cells with 10−5 mol/L BRL37344 treatment; BRL37344 group: lipid-loaded RAW264.7 cell treated with 10−6 mol/L BRL37344 only; and SR59230A group: lipid-loaded RAW264.7 cell treated with 10−6 mol/L SR59230A only. Statistically significant differences are indicated (compared with control group, *P<0.01).
Abbreviations: CE, cholesteryl ester; FC, free cholesterol; TC, total cholesterol.