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. 2017 Mar 9;12(3):e0173417. doi: 10.1371/journal.pone.0173417

Fig 3. Morphology of MCs in burn healing process.

Fig 3

(a) MCs (arrows), most intact in Normal Skin (N). (b) C3 and (c) SDP3 with few MCs and mostly degranulated. (d) C14 (e) SDP14, MCs degranulated, mainly in control group. (f) C21, numerous intact MCs. Details of intact (a and f) and degranulated MCs (b and c). Staining: Toluidine Blue. Bars 50 μm. Quantification of MCs: (g) numerous intact MCs in N and C21 and (h) degranulated MCs in SDP7 and in both groups on day 14. (i) Differences between quantification of MCs evidenced by Toluidine blue or Safranin-O, with few MCs S-O+ (MCs histamine storage) in SDP7, C14 and SDP14 compared to the MC TB+ (total MCs in the tissue). (j) MC stained with Safranin-O (MC S-O+); (k) reactive MC for tryptase (MCT) and (l) chymase (MCC); Bars 50 μm. Heterogeneity of MCs, showing many MCTs (m) in N and numerous MCCs (n) in C21. Values are presented as mean ± S.E.M. (n = 5/group). * p<0.05 vs N and γ p<0.001 vs C14.