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. 2017 Mar 9;13(3):e1006219. doi: 10.1371/journal.ppat.1006219

Fig 7. Rhesus adaptive immune cell proliferative responses following ZIKV infection.

Fig 7

Total peripheral blood mononuclear cells were analyzed by flow cytometry for the presence of T and B cell proliferative responses following infection. T cells were identified by staining with antibodies directed against the cellular markers CD3, CD4, CD8β, CD95, CD28, CD127 and for intracellular levels of Ki67 (proliferation marker) to assess changes the proliferation of A) CD4+ central memory T cells; B) CD8+ central memory T cells; C) CD4+ effector memory T cells; and D) CD8+ effector memory T cells. B cells were stained with antibodies directed against CD3, CD20, CD27, IgD and HLA-DR as well as Ki67 in order to compare the proliferative responses in E) naïve B cell; F) memory B cells; and G) marginal-zone like B cells. The percentage of actively proliferating cells (Ki67+) was calculated using FlowJo and the data was graphed in GraphPad Prism v6 software.