Skip to main content
. 2017 Feb 27;13(2):e1006226. doi: 10.1371/journal.ppat.1006226

Fig 5. Microbial exposure enhances TF HIV-1 infection and CD4+ T cell death in the LPAC model.

Fig 5

After spinoculation with the TF HIV-1 CH040.c strain, LPMCs were resuspended in media containing or not containing heat-killed Prevotella stercorea (P.s.) at a 2.5 bacteria: 1 LPMC ratio. Supernatants and cells were analyzed at 6 dpi. (A) Infectious titers. Supernatants were evaluated for infectious HIV-1 titers using TZM-bl reporter cells. Log-transformed firefly luciferase values are shown. (B) CD4+ T cell depletion. The difference in the absolute number of CD3+CD8- T cells between HIV-1 infected and uninfected (mock) LPMC cultures were calculated. Mock controls for CH040.c only was not exposed to P. stercorea, while the mock controls for CH040.c+P.s. were exposed to P. stercorea. For both panels, each connected dot corresponds to a different LPMC donor (n = 9 donors). Data were analyzed using a paired 2-tailed Student’s t test. ***p<0.001.