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. 2017 Feb 27;13(2):e1006237. doi: 10.1371/journal.ppat.1006237

Fig 4. Transgenic microbes secreting uNLPs protect tomato against Meloidogyne incognita and Globodera pallida.

Fig 4

(A) Nine independent Chlamydomonas reinhardtii transformants secreting two distinct nematode neuropeptides (Mi-NLP-9f and Mi-NLP-15b) significantly inhibited the ability of M. incognita J2s to infect tomato plants, with up to 90% protection. (B) Bacillus subtilis cultures secreting either Mi-NLP-15b or Mi-NLP-40 also conferred significant protection against M. incognita J2 invasion. (C) C. reinhardtii transformants secreting Gp-NLP-15b (identical to Mi-NLP-15b) significantly inhibited the ability of G. pallida J2s to invade tomato plants. (D) B. subtilis cultures secreting Gp-NLP-15b also protected tomato plants from G. pallida J2 invasion. Data shown represents mean±SEM. *, P<0.05; **, P<0.01; ***, P<0.001 (One-way ANOVA & Fisher’s LSD; Graphpad Prism 6).