Figure 3.

Chromatin Modifier Overexpression Alleviates Resistance to Transcriptional Reprogramming

(A) Schematic representation shows the nuclear transplantation strategy used to test the sensitivity of resistant genes to chromatin modifiers.

(B) Western blot analysis of modified histones on chromatin recovered 48 hr after nuclear transplantation to oocyte expressing single or multiple chromatin modifiers is shown.

(C) Summary shows the 12 chromatin configurations tested in the OOC transcriptional reprogramming assay.

(D) Boxplot shows the log fold change between resistant gene expression in MEF-NT in various chromatin configurations to that of ES-NT.

(E) Number of resistant genes as judged by differential gene expression analysis between RNA-seq data from ES-NT and MEF-NT in various chromatin configurations (FDR < 0.05, two experiments, n = 32 NT samples per conditions). See also Figure S3 and Table S3.