Figure 2.

Loss of Cdc42 in the Melanoblast Lineage Delays Dorsal-to-Ventral Population of the Skin after E11.5

Photos show X-gal-stained Ctr and Cdc42 f/f embryos at various stages as indicated. In all panels, Ctr indicates Cdc42 wt/wt; DCT::LacZ controls, whereas Cdc42 f/f indicates Cdc42 f/f; Tyr::CreB+; DCT::LacZ.

(A) E11.5 embryos; dashed lines denote the quantification area. Scale bar, 1 mm.

(B) Number of melanoblasts in E11.5 embryos.

(C) E13.5 embryos; dashed lines denote melanoblast progress. Scale bar, 1 mm.

(D) Quantification area example.

(E) E13.5 embryos with quantification area. Scale bar, 1 mm.

(F) Melanoblasts from box 1 (most dorsal) to box 6 (ventral) at E13.5.

(G) E15.5. Scale bar, 1 mm. The dashed line indicates the border where the furthest migrating melanoblasts from the neural tube have appeared.

(H) Melanoblasts counted in boxes 1–6 as shown in (D) at E15.5.

(I) Zoom-in of E15.5 embryos. Scale bar, 200 μm.

Mean ± SEM. N ≥ 4 embryos from three litters. Kruskal-Wallis one-way ANOVA test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; n.s., not significant. See also Figure S2.