Figure 2.

Purification and Nuclease Activities of the SMX Tri-nuclease

(A) Purification scheme for SMX from baculovirus-infected insect cells.

(B) SDS-PAGE gel showing SMX stained with SYPRO Ruby. Asterisk denotes co-purifying tubulin α/β.

(C) The indicated DNA substrates (50 nM), 5′-³²P end-labeled on one oligonucleotide (indicated with an asterisk), were incubated with purified SMX (0.5 nM) for the indicated times. Reaction products were analyzed by neutral PAGE.

(D) Quantification of (C). Product formation is expressed as a percentage of total radiolabeled DNA. Results are presented as the mean of at least three independent experiments ± SEM.

See also Figures S2 and S3 and Table S1.