Skip to main content
. Author manuscript; available in PMC: 2017 Aug 2.
Published in final edited form as: Nature. 2017 Feb 2;543(7644):248–251. doi: 10.1038/nature21428

Extended Data Figure 1. Design and characterization of ZIKV prM-E mRNA.

Extended Data Figure 1

(a) The ZIKV mRNA encodes the signal peptide (SP) from MHC class II and prM and E glycoproteins from ZIKV H/PF/2013. (b) mRNA was transfected into 293T cells (n=3), human DC (n=3), or murine DC (n=2). E protein expression in cell lysate and supernatant was probed by Western blot, using firefly luciferase-encoding mRNA-transfected cells as a negative control. (c) ZIKV mRNA supernatant from transfected 293T cells was characterized by ultracentrifugation in the presence and absence of 0.5% Triton X-100, followed by Western blot of input (IN), pellet (P), and final supernatant (S) fractions (n=3).