Extended Data Fig. 8. Functionality of AT^lo and AT^hi old HSC subsets.

a, Scheme for primary and secondary transplantations (tplx) of AT^lo or AT^hi young and old HSCs. b, Transplantation of AT^lo and AT^hi oHSC subsets with 15% GFP-LC3 high/low expression cutoff showing donor chimerism (left) and lineage distribution (center) in PB, and HSC chimerism (right) at the indicated times post-tplx in primary (top row) and secondary (bottom row) recipients. c, GFP-LC3 levels in AT^lo and AT^hi oHSCs before transplantation, and in primary and secondary recipients. Results are expressed relative to GFP-LC3 levels in young HSCs (± S.E.M.). d, Transplantation of AT^lo and AT^hi yHSC subsets with 33% GFP-LC3 high/low expression cutoff showing donor chimerism (left) and lineage distribution (center) in PB, and HSC chimerism (right) at the indicated times post-tplx in primary recipients. Data are mean ± S.D. except when indicated. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.