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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Nature. 2017 Mar 1;543(7644):205–210. doi: 10.1038/nature21388

Figure 3. Loss of autophagy and aging cause metabolic activation in HSCs.

Figure 3

a, OXPHOS levels measured by oxygen consumption rates (OCR) in freshly isolated, quiescent HSCs (qHSC, same as yHSC in e) or activated HSCs (aHSC; 21hr +cyt culture); oligo: oligomycin; R&A: rotenone and antimycin; min: minute. b, glucose uptake in Cnt and cKO HSCs cultured as indicated. Results are expressed relative to 0hr Cnt HSC levels. c, ATP and NADH levels in the indicated HSC populations (± S.D.). d, OXPHOS levels in Cnt and cKO LSK cells. e, OXPHOS levels in yHSCs and oHSCs. Data are mean ± S.E.M. except when indicated. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.