Figure 7.

Characterization of synthetic mutants with partial deletion of exons 4 and 5. (a) Probasin-based reporter was used to evaluate transactivation activities of a series of deletion mutants. Luciferase activities were measured as described in Figure 2a. All individual data points were plotted. Similar results were obtained from three independent experiments. (b) Western blot analysis confirmed comparable expression of FLAG AR constructs. The common cell lysates of v6es sample were loaded on two different gels loaded with the samples including F771X and those including V747X as the control to compare the intensities of the bands on the different gels. The vertical dotted line indicates western blot image cropped from the same gel. (c) Binding affinity of deletion mutants to HSP90. FLAG-tagged AR mutants expressed in M12 cells were subjected to co-IP assay as described in Figure 4. (d) Subcellular localization of AR deletion mutants FLAG-tagged constructs were evaluated in M12 as described in Figure 5. Localization was classified following the similar criteria. N and N>C, white bar; N=C, green; N<C and C, red. Three hundred cells were assessed in each well. Similar results were obtained from three independent experiments.