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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mech Dev. 2017 Jan 10;143:32–41. doi: 10.1016/j.mod.2017.01.003

Figure 4. Decreased Wnt signaling causes an increase in 1st arch PM cells.

Figure 4

(A, B) Tg(tcf21:nucEGFP) control sibling embryos and embryos with decreased Wnt signaling at the SP stage. Decreased Wnt signaling causes enlargement of the 1st arch AM muscle (boxes). Sarcomeric myosin (MHC; red). NucGFP (green). (B, E) MHC alone of AM muscles. (C, F) NucEGFP alone of AM. (G) Graph depicting quantification of nuclei in the AM muscles. Decreasing Wnt signaling at the SP stage produces a modest, but significant increase in AM muscle nuclei. Control embryos n=15, Tg(hsp70l:dkk1-GFP) embryos n=7. Asterisks in all graphs indicate p<0.05. Error bars in all graphs indicate S.E.M. Scale bar in A indicates 100 µm. Scale bar in B indicates 50 µm.