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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mech Dev. 2017 Jan 10;143:32–41. doi: 10.1016/j.mod.2017.01.003

Figure 6. Manipulation of Wnt signaling affects the overlap of nkx2.5+ and tcf21+ domains within the ALPM.

Figure 6

(A–H) Tg(nkx2.5:ZsYellow) control sibling embryos and embryos with increased and decreased Wnt signaling at the SP stage. (A,B,E,F) Two-color ISH of zsyellow (nkx2.5; brownish red) and tcf21 (blue) at the 14s and 18s stages shows expansion of the lateral nkx2.5+ domains into the anterior tcf21+ domain after increased Wnt signaling. Numbers in E indicate the three tcf21+ fields depicted in Figure 5. (C,D,G,H) Decreasing Wnt signaling prior to gastrulation causes a decrease in the overlap of nkx2.5 and tcf21 at the 14s and 18s stages. Significant overlap between nkx2.5 and tcf21 could not be detected at the 14s stage in embryos when with Wnt signaling was inhibited prior to gastrulaiton. Images are dorsal views with anterior to left. Brackets indicate overlap of nkx2.5+ and tcf21+ domains. For each group >15 embryos were examined. Scale bar indicates 100 µm.