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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Microbiol. 2017 Feb 7;103(6):931–957. doi: 10.1111/mmi.13613

Fig. 5.

Fig. 5

D39 Δcps ΔgpsB phpP(G229D) suppressor strains grow similarly to wild-type parent strains, but form slightly rounder, smaller-sized cells. A) Representative growth curves of the originally isolated ΔgpsB sup1 (phpP(G229D)) (Table 2, line 1) and reconstructed strain (Fig. 4B). Strain 1, D39 Δcps wild-type parent (IU1945); strain 2, original ΔgpsB sup1 strain (D39 Δcps ΔgpsB phpP(G229D); IU6442, Table 2, line 1); strain 3, E46 used in reconstruction (D39 Δcps ΔbgaA::Pc-erm; Fig. 4B); strain 4, reconstructed suppressor (D39 Δcps ΔgpsB phpP(G229D) ΔbgaA::Pc-erm; IU11221; Fig. 4B). Doubling times were calculated as described for Figure 1. B) Representative images of live cells at OD620 ≈ 0.1–0.2 from growth curves in panel A. C) Length, width, aspect ratio, and cell volume relative to the median value of IU1945 for each strain listed in panel A. Over 50 cells were measured per strain in two experimental replicates. Scale bar = 1 micron.