Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 10;5:12. doi: 10.3389/fchem.2017.00012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Czapiński, Kiełbus, Kałafut, Kos, Stepulak and Rivero-Müller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Chemical-induced dimerization and protein stabilization. (A) The FKBP/FRB system based on rapamycin-induced protein dimerization. This system brings two protein, or two complementing fragments, together, when one is fused to FKBP and the second to FRB in the presence of rapamycin (Rapa). (B) The fusion of a destabilizing domain (DD) to a protein of interest (POI) leads to its rapid degradation by the proteasome. However, in the presence of a suitable ligand [Shield1 for FKBP or Trimethoprim (TMP) for DHFR] the DD is stabilized and the POI accumulates in the cell.